摘  要：利用CN-誘導(dǎo)的邁克爾加成反應(yīng)阻斷探針的分子內(nèi)電荷轉(zhuǎn)移過(guò)程（ICT），成功設(shè)計(jì)合成一種新型的熒光比率型CN-熒光探針。該探針對(duì)CN-具有顯著的熒光比率響應(yīng)，其熒光光譜藍(lán)移128 nm。相對(duì)于其他干擾離子，探針對(duì)CN-具有較高的選擇性和專(zhuān)一性。通過(guò)以上實(shí)驗(yàn)為監(jiān)測(cè)CN-濃度提供一個(gè)簡(jiǎn)單有效并具有較高靈敏度的分析方法。

關(guān)鍵詞：熒光探針；比率型；分子內(nèi)電荷轉(zhuǎn)移；香豆素衍生物

文獻(xiàn)標(biāo)志碼：A       文章編號(hào)：1674-5124（2015）12-0045-04

Synthesis and application of ratiometric fluorescent probe for CN- determination

CHEN Song， HOU Peng， WANG Jing， LIU Lei， WANG Xin

（College of Pharmacy，Qiqihar Medical University，Qiqihar 161006，China）

Abstract: It reports a new type of CN- fluorescence ratiometric fluorescent probe by using the CN- induced Michael addition reaction to block intermolecular charge transfer process（ICT）. The probe displayed remarkable fluorescence ratio towards CN-. With the addition of CN-， the fluorescence spectrum of the probe blue shifted by 128 nm. The probe exhibited high selectivity and sensitivity towards CN- over other interfering ions. based on the result， it provides a simple， effective and high sensitivity analysis method for monitoring the concentration of CN-.

Keywords: fluorescence probe； ratiometric； ICT； coumarin derivative